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bt fgfb rhvegf r d systems  (R&D Systems)


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    R&D Systems bt fgfb rhvegf r d systems
    Bt Fgfb Rhvegf R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf+basic+protein+r/Recombinant+Human+FGF+basic%2FFGF2+Protein%2C+CF/pm41564860-638-185-187
    Average 95 stars, based on 212 article reviews
    bt fgfb rhvegf r d systems - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Mouse C57BL/6 Vital River Laboratory Animal Technology Cell line (Homo-sapiens) 293FT cells Thermo Fisher Scientific Cat# R70007 Cell line (Homo-sapiens) H1 ES cells WiCell Research Institute Cat# WA01 Cell line (Homo-sapiens) Human Pulmonary Fibroblasts National Infrastructure of Cell Line Resource Cat# CCC-HPF-1 (PUMC, Beijing) Transfected construct (Homo-sapiens) p2k7-EF1alpha-NR5A1 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-GATA4 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-SOX9 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-WT1 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-DMRT1 This paper Lentiviral construct to express target gene Transfected construct p2k7-EF1alpha-luciferin This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-CX43 This paper Lentiviral construct to express target gene Recombinant DNA reagent p2k7-AMH-GFP This paper Lentiviral construct for AMH reporter Recombinant DNA reagent pX335-U6-Chimeric_ BB-CBh-hSpCas9n(D10A) Addgene #42335 Expressing Cas9 and gRNA (Cong et al., 2013) Biological sample (Homo-sapiens) Primary adult human Sertoli cells Shanghai Jiao Tong University Freshly isolated Antibody anti-DAZL (Mouse polyclonal) AbD Serotec Cat#: MCA2336; RRID: AB_2292585 IF(1:50) Antibody anti-NuMA (Rabbit polyclonal) Abcam Cat#: Ab97585; RRID: AB_GR27454-16 IF(1:100) Antibody anti-human KRT18 (Rabbit polyclonal) Proteintech 10830–1-AP IF(1:100) Antibody anti-SOX9 (Rabbit monoclonal) Abcam Cat#: Ab170660; RRID: AB_GR155689-1 IF(1:100) Continued on next page Liang et al. eLife 2019;8:e48767. .. DOI: https://doi.org/10.7554/eLife.48767 18 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-human CX43 (Rabbit polyclonal) Cell Sigaling Cat#: 3512S IF(1:100) Antibody anti-human DMRT1 (Rabbit polyclonal) Abcam Cat#: Ab1786 IF(1:100) Antibody anti-human WT1 (Rabbit polyclonal) Proteintech Cat#: 12609–1-AP IF(1:100) Antibody anti-human GATA4 (Rabbit polyclonal) Proteintech Cat#: 19530–1-AP IF(1:100) Antibody anti-human NR5A1 (Rabbit polyclonal) Proteintech Cat#: 18658–1-AP IF(1:100) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#: A-21202; RRID: AB_141607 IF(1:1000) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#: A-31572; RRID: AB_1567203 IF(1:1000) Strain, strain background (Escherichia coli) TransStbl3 Chemically Competent Cell TransGen Biotech Cat#: CD521 Peptide, recombinant protein bFGF, Recombinant Human FGF basic Protein R and D Systems Cat#: 233-FB-CF Chemical compound, drug G418, Geneticin Thermo Fisher Scientific Cat#: 10131035 Chemical compound, drug Blasticidin Thermo Fisher Scientific Cat#: R21002 Software, algorithm, website ImageJ NIH https://imagej.nih.gov/ij/ Software, algorithm, website FlowJo (v10.3) BD https://www.flowjo.com Software, algorithm, website Prism7 (v7.0 a) Graphpad Software https://www.graphpad.com/ scientific-software/prism/ Software, algorithm DAVID Bioinformatics Resources (v6.8; GO) https://david.ncifcrf.gov Software, algorithm, website Tophat2/cufflinks (Kim et al., 2013) http://ccb.jhu.edu/ software/tophat Software, algorithm, website R (v3.4.1; PCA, cluster and DEG) https://www. .. R-project.org Other DAPI stain Invitrogen D1306 (1 mg/mL) Experimental model and subject details Animal Care and Use C57BL/6 mice were purchased from Vital River Laboratory Animal Technology Co., Ltd (Beijing, China).

    Software:

    Article Title: Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Mouse C57BL/6 Vital River Laboratory Animal Technology Cell line (Homo-sapiens) 293FT cells Thermo Fisher Scientific Cat# R70007 Cell line (Homo-sapiens) H1 ES cells WiCell Research Institute Cat# WA01 Cell line (Homo-sapiens) Human Pulmonary Fibroblasts National Infrastructure of Cell Line Resource Cat# CCC-HPF-1 (PUMC, Beijing) Transfected construct (Homo-sapiens) p2k7-EF1alpha-NR5A1 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-GATA4 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-SOX9 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-WT1 This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-DMRT1 This paper Lentiviral construct to express target gene Transfected construct p2k7-EF1alpha-luciferin This paper Lentiviral construct to express target gene Transfected construct (Homo-sapiens) p2k7-EF1alpha-CX43 This paper Lentiviral construct to express target gene Recombinant DNA reagent p2k7-AMH-GFP This paper Lentiviral construct for AMH reporter Recombinant DNA reagent pX335-U6-Chimeric_ BB-CBh-hSpCas9n(D10A) Addgene #42335 Expressing Cas9 and gRNA (Cong et al., 2013) Biological sample (Homo-sapiens) Primary adult human Sertoli cells Shanghai Jiao Tong University Freshly isolated Antibody anti-DAZL (Mouse polyclonal) AbD Serotec Cat#: MCA2336; RRID: AB_2292585 IF(1:50) Antibody anti-NuMA (Rabbit polyclonal) Abcam Cat#: Ab97585; RRID: AB_GR27454-16 IF(1:100) Antibody anti-human KRT18 (Rabbit polyclonal) Proteintech 10830–1-AP IF(1:100) Antibody anti-SOX9 (Rabbit monoclonal) Abcam Cat#: Ab170660; RRID: AB_GR155689-1 IF(1:100) Continued on next page Liang et al. eLife 2019;8:e48767. .. DOI: https://doi.org/10.7554/eLife.48767 18 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-human CX43 (Rabbit polyclonal) Cell Sigaling Cat#: 3512S IF(1:100) Antibody anti-human DMRT1 (Rabbit polyclonal) Abcam Cat#: Ab1786 IF(1:100) Antibody anti-human WT1 (Rabbit polyclonal) Proteintech Cat#: 12609–1-AP IF(1:100) Antibody anti-human GATA4 (Rabbit polyclonal) Proteintech Cat#: 19530–1-AP IF(1:100) Antibody anti-human NR5A1 (Rabbit polyclonal) Proteintech Cat#: 18658–1-AP IF(1:100) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#: A-21202; RRID: AB_141607 IF(1:1000) Antibody donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat#: A-31572; RRID: AB_1567203 IF(1:1000) Strain, strain background (Escherichia coli) TransStbl3 Chemically Competent Cell TransGen Biotech Cat#: CD521 Peptide, recombinant protein bFGF, Recombinant Human FGF basic Protein R and D Systems Cat#: 233-FB-CF Chemical compound, drug G418, Geneticin Thermo Fisher Scientific Cat#: 10131035 Chemical compound, drug Blasticidin Thermo Fisher Scientific Cat#: R21002 Software, algorithm, website ImageJ NIH https://imagej.nih.gov/ij/ Software, algorithm, website FlowJo (v10.3) BD https://www.flowjo.com Software, algorithm, website Prism7 (v7.0 a) Graphpad Software https://www.graphpad.com/ scientific-software/prism/ Software, algorithm DAVID Bioinformatics Resources (v6.8; GO) https://david.ncifcrf.gov Software, algorithm, website Tophat2/cufflinks (Kim et al., 2013) http://ccb.jhu.edu/ software/tophat Software, algorithm, website R (v3.4.1; PCA, cluster and DEG) https://www. .. R-project.org Other DAPI stain Invitrogen D1306 (1 mg/mL) Experimental model and subject details Animal Care and Use C57BL/6 mice were purchased from Vital River Laboratory Animal Technology Co., Ltd (Beijing, China).



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